Lab Experimental Protocols

Lab Experimental Protocols are available on this page. Contact Rick.Maizels@glasgow.ac.uk for any that you think we should add here.

Parasite Life Cycles – Our Current Protocols

Life cycle of Heligmosomoides polygyrus – PDF

Life cycle of Nippostrongylus brasiliensis – PDF

Preparation of HES, H. polygyrus ES products – PDF

Mucosal Immunology

Cell isolation from Peyer’s Patches – PDF

Induction of Foxp3+ Regulatory T cells

In vitro culture conditions for Treg induction – PDF

Organoid Culture

Marta Campillo’s STAR Protocol for mouse small intestinal organoid cultures.

Molecular Parasitology

In 1988 we assembled the Molecular Parasitology Lab Manual 1988. After 35 years it is surprising how many protocols remain in use today ! Below are some more up-to-date ones…

Production of polyclonal antibodies by Alum Immunisation – PDF